Delivering the second revolution in site-specific nucleases
نویسندگان
چکیده
منابع مشابه
Delivering the second revolution in site-specific nucleases
Viruses have been used to deliver two types of site-specific nucleases into cells for targeted gene editing.
متن کاملMonomeric site-specific nucleases for genome editing.
Targeted manipulation of complex genomes often requires the introduction of a double-strand break at defined locations by site-specific DNA endonucleases. Here, we describe a monomeric nuclease domain derived from GIY-YIG homing endonucleases for genome-editing applications. Fusion of the GIY-YIG nuclease domain to three-member zinc-finger DNA binding domains generated chimeric GIY-zinc finger ...
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Inclusion of the woodchuck hepatitis virus post-transcriptional response element (WPRE) in the 3’ UTR of mRNA encoding zinc-finger or TALE nucleases results in up to a fifty-fold increase in nuclease expression and a several-fold increase in nuclease-modified chromosomes. Significantly, this increase is additive with the enhancement generated by transient hypothermic shock. The WPRE-mediated im...
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The fields of molecular genetics, biotechnology and synthetic biology are demanding ever more sophisticated molecular tools for programmed precise modification of cell genomic DNA and other DNA sequences. This review presents the current state of knowledge and development of one important group of DNA-modifying enzymes, the site-specific recombinases (SSRs). SSRs are Nature's 'molecular machine...
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ژورنال
عنوان ژورنال: eLife
سال: 2014
ISSN: 2050-084X
DOI: 10.7554/elife.02904